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The cells were seeded at a density of 1.0x10 4 cells in 0.1% collagen pre-coated 96-well plate, and used for the ethanol exposure experiments after being cultured overnight

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The DNA was extracted and purified from the sample using the DNeasy PowerSoil Pro Kit (Qiagen Italia, Milan, Italy)

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MMD collaborates with ACSL4 and MBOAT7 to promote polyunsaturated phosphatidylinositol remodeling and susceptibility to ferroptosis